General Cell Based Assays
Eurofins Discoverx cAMP Hunter™ Human RXFP3 Gi Cell-Based Assay Kit (CHO-K1)
The cAMP Hunter™ Human RXFP3 Gi Stable Cell Line Assay (CHO-K1) contains a stable clonal cell line used to measure RXFP3 (GPCR) activity via decreased levels of intracellular cAMP. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
Eurofins Discoverx AssayComplete Freezing Reagent F6, 2 x 50. (92-5106FR)
AssayComplete Freezing Reagent F6 is an optimized freezing reagent for cryopreservation of Eurofins DiscoverX cell lines. This reagent ensures optimal cell viability and assay performance upon thawing. This product is provided as 2 bottles of 50 mL reagent each. Please refer to cell line specific datasheet to determine the recommended freezing reagent. For detailed product information, User manual and datasheet, visit www.discoverx.com/reagents.
Cayman Chemical Cholesterol Assay Horseradish Peroxidase, 1 ea
Cholesterol Assay Horseradish Peroxidase, 1 ea
Cayman Chemical Cholesterol Assay Horseradish Peroxidase, 5 ea
Cholesterol Assay Horseradish Peroxidase, 5 ea
Eurofins Discoverx PathHunter eXpress HCRTR1 CHO-K1 β-Arrestin GPCR Assay, 2-Plate Kit. (93-0258E2CP0M)
The PathHunter HCRTR1 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. orexin A) based activation of HCRTR1 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged HCRTR1 and EA-tagged β-Arrestin-2. Activation of HCRTR1 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Meso Scale Discovery R-PLEX Human Enolase 2 ASY
The R-PLEX Human Enolase 2 Assay Kit from Meso Scale Discovery is used to quantitatively measure enolase 2 in human serum plasma and cell culture supernatants It utilizes an electrochemiluminescent detection method and provides high sensitivity and broad dynamic range The assay takes approximately 4 hours to complete and requires an MSD instrument such as QuickPlex QuickPlex SQ 120 or QuickPlex SQ 240 Each kit provides reagents for one 96-well plate which can assay 40 samples in duplicate
Eurofins Discoverx cAMP Hunter™ Human HRH3 (H3) Gi Cell-Based Assay Kit (CHO-K1)
The cAMP Hunter™ Human HRH3 (H3) Gi Stable Cell Line Assay (CHO-K1) contains a stable clonal cell line used to measure HRH3 (GPCR) activity via decreased levels of intracellular cAMP. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
Montana Molecular Borealis Arrestin D1R Assay - 10mL
Measure arrestin signaling kinetics in real time with the live cell, fluorescent Borealis arrestin - D1 Dopaine Receptor (D1R) assay. Add the unmodified D1 receptor and Borealis sensor to cells and measure robust signals the next day on an automated plate reader or fluorescence imaging system. This assay is genetically encoded and packaged in BacMam for efficient transduction in most cell types - including uniform and near 100% efficiency in HEK293 cells as well as expression in many primary or iPSC-derived cells. The kit includes the arrestin sensor BacMam, D1R BacMam, 500mM sodium butyrate in H2O, and a positive control receptor and agonist (AT1R BacMam and 5mM Angiotensin II in H2O). Borealis kits for several other receptors are also available, and larger-sized kits are available on request. Other Montana Molecular tools including the cADDis cAMP sensor can be used alongside this assay.
Eurofins Discoverx PathHunter Human VIPR1 β-Arrestin Cell-Based Assay Kit (CHO-K1)
The PathHunter Human VIPR1 β-Arrestin Stable Cell Line Assay (CHO-K1) contains a stable clonal cell line used to measure VIPR1 (GPCR) activity via recruitment of β-Arrestin2. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
Meso Scale Discovery U-PLEX Mouse TNF-RI ASY
The Meso Scale Discovery U-PLEX Mouse TNF RI Assay Kit is a multiplex immunoassay that measures mouse tumor necrosis factor receptor I (TNF RI) in serum plasma and cell culture supernatants It uses electrochemiluminescence (ECL) detection technology and provides high sensitivity up to 5 logs of dynamic range and low sample volume requirements This assay kit includes a 96-well plate coated with capture antibodies detection antibodies labeled with an ECL tag calibrators and buffer for sample dilution It can test up to 80 samples in duplicate and has a testing time of 3 5 hours The assay has high specificity and shows minimal cross-reactivity
Eurofins Discoverx PathHunter Human EGFR/EGFR Dimerization Cell-Based Assay Kit (U2OS)
The PathHunter Human EGFR/EGFR Dimerization Stable Cell Line Assay (U2OS) measures ligand-induced dimerization of EGFR/EGFR. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
Cayman Chemical ML-148 asy Reagent 25uL
A C-glycoside flavonoid with diverse biological activities reduces H